PUMA antibody (C-Term)

Catalog No. ABIN7216706

This anti-PUMA antibody is a Rabbit Polyclonal antibody detecting PUMA in WB and ELISA. Suitable for Human, Rat and Mouse.

Quick Overview for PUMA antibody (C-Term) (ABIN7216706)

Target: PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PUMA antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-PUMA Antibody

Binding Specificity: C-Term

Purpose: PUMA Polyclonal Antibody

Specificity: PUMA Polyclonal Antibody detects endogenous levels of PUMA protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the C-terminal region of human PUMA

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:10000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for PUMA

Target: PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

Alternative Name: PUMA

Background: Rabbit Anti-PUMA Polyclonal Antibody,BBC3, PUMA, Bcl-2-binding component 3, JFY-1, p53 up-regulated modulator of apoptosis,BBC3 encodes a member of the BCL-2 family of proteins. This family member belongs to the BH3-only pro-apoptotic subclass. BCL2 binding component 3 cooperates with direct activator proteins to induce mitochondrial outer membrane permeabilization and apoptosis. It can bind to anti-apoptotic Bcl-2 family members to induce mitochondrial dysfunction and caspase activation. Because of its pro-apoptotic role, BBC3 is a potential drug target for cancer therapy and for tissue injury. Alternative splicing results in multiple transcript variants.,Bcl-2-binding component 3

Molecular Weight: observerd band 23kDa

Gene ID: 27113

UniProt: Q9BXH1

Pathways: p53 Signaling, Positive Regulation of Endopeptidase Activity