CD22 antibody (pTyr807)

Catalog No. ABIN7225948

This anti-CD22 antibody is a Rabbit Polyclonal antibody detecting CD22 in WB, ELISA and IHC (p). Suitable for Human.

Quick Overview for CD22 antibody (pTyr807) (ABIN7225948)

Target: CD22 (CD22 Molecule (CD22))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD22 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CD22 Antibody

Binding Specificity: pTyr807

Purpose: CD22 (phospho Tyr807) Polyclonal Antibody

Specificity: Phospho-CD22 (Y807) Polyclonal Antibody detects endogenous levels of CD22 protein only when phosphorylated at Y807.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human CD22 Phospho-Tyr807

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:40000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for CD22

Target: CD22 (CD22 Molecule (CD22))

Alternative Name: CD22

Background: Rabbit Anti-CD22 (phospho Tyr807) Polyclonal Antibody,CD22, SIGLEC2, B-cell receptor CD22, B-lymphocyte cell adhesion molecule, BL-CAM, Sialic acid-binding Ig-like lectin 2, Siglec-2, T-cell surface antigen Leu-14, CD antigen CD22,CD22, also known as Siglec-2 (sialic acid binding Ig-like lectin 2) or BL-CAM (B-lymphocyte cell adhesion molecule), is a 130-140 kDa, B-cell restricted, type I transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. The expression of CD22 is developmentally regulated. It is expressed at low levels in the cytoplasm of pro-B and pre-B cells and present on the cell surface only at mature stages of B-cell differentiation. Cell surface expression is lost during terminal differentiation into plasma cell and after B-cell activation. CD22 is an inhibitory receptor for B-cell receptor (BCR) signalling, preferentially binds to alpha-2,6-linked sialic acid and mediates B-cell B-cell interactions. It plays a crucial role in activation and differentiation of the B-cell.,B-cell receptor CD22

Molecular Weight: observerd band 95kDa

Gene ID: 933

UniProt: P20273