CDC25C antibody

Catalog No. ABIN7214102

This Rabbit Polyclonal antibody specifically detects CDC25C in WB, IHC, ELISA and IF. It exhibits reactivity toward Human and Mouse.

Quick Overview for CDC25C antibody (ABIN7214102)

Target: CDC25C (Cell Division Cycle 25 Homolog C (S. Pombe) (CDC25C))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDC25C antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-CDC25C Antibody

Purpose: Rabbit Anti-Cdc25C Polyclonal Antibody

Specificity: Cdc25C Polyclonal Antibody detects endogenous levels of Cdc25C protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human Cdc25C around the non-phosphorylation site of S216

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:40000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for CDC25C

Target: CDC25C (Cell Division Cycle 25 Homolog C (S. Pombe) (CDC25C))

Alternative Name: Cdc25C

Background: CDC25C, M-phase inducer phosphatase 3, Dual specificity phosphatase Cdc25CCDC25C encodes a conserved protein (cell division cycle 25C) that plays a key role in the regulation of cell division. Cell division cycle 25C directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It also suppresses p53-induced growth arrest. Multiple alternatively spliced transcript variants of CDC25C have been described.

Molecular Weight: 53kD

Gene ID: 995

UniProt: P30307

Pathways: Cell Division Cycle, M Phase