CDC25C antibody (pThr48)

Catalog No. ABIN7226182

The Rabbit Polyclonal anti-CDC25C antibody is suitable to detect CDC25C in samples from Human, Mouse and Rat. It has been validated for WB, IHC, ELISA and IF.

Quick Overview for CDC25C antibody (pThr48) (ABIN7226182)

Target: CDC25C (Cell Division Cycle 25 Homolog C (S. Pombe) (CDC25C))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDC25C antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-CDC25C Antibody

Binding Specificity: pThr48

Purpose: Rabbit Anti-Cdc25C (phospho Thr48) Polyclonal Antibody

Specificity: Phospho-Cdc25C (T48) Polyclonal Antibody detects endogenous levels of Cdc25C protein only when phosphorylated at T48.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human Cdc25C Phospho-Thr48

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:5000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for CDC25C

Target: CDC25C (Cell Division Cycle 25 Homolog C (S. Pombe) (CDC25C))

Alternative Name: Cdc25C

Background: CDC25C, M-phase inducer phosphatase 3, Dual specificity phosphatase Cdc25CCDC25C (cell division cycle 25C) encodes a conserved protein that plays a key role in the regulation of cell division. The encoded protein directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It also suppresses p53-induced growth arrest. Multiple alternatively spliced transcript variants of CDC25C have been described.

Molecular Weight: 80kD

Gene ID: 995

UniProt: P30307

Pathways: Cell Division Cycle, M Phase