CBX3 antibody

Catalog No. ABIN7212344

This Mouse Monoclonal antibody specifically detects CBX3 in WB, IHC and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for CBX3 antibody (ABIN7212344)

Target: CBX3 (Chromobox Homolog 3 (CBX3))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CBX3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: 3B9

Product Details anti-CBX3 Antibody

Purpose: Mouse Anti-HP-1γ Mouse Monoclonal Antibody (3B9)

Specificity: The antibody detects endogenous HP-1γ protein.

Purification: The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Recombinant Protein of HP-1gamma

Isotype: IgG1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,IHC 1:50-300,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for CBX3

Target: CBX3 (Chromobox Homolog 3 (CBX3))

Alternative Name: HP-1gamma

Background: Chromobox protein homolog 3, HECH, Heterochromatin protein 1 homolog gamma, HP1 gamma, Modifier 2 proteinAt the nuclear envelope, the nuclear lamina and heterochromatin are adjacent to the inner nuclear membrane. The protein encoded by CBX3 (chromobox 3) binds DNA and is a component of heterochromatin. This protein also can bind lamin B receptor, an integral membrane protein found in the inner nuclear membrane. The dual binding functions of the encoded protein may explain the association of heterochromatin with the inner nuclear membrane. This protein binds histone H3 tails methylated at Lys-9 sites. This protein is also recruited to sites of ultraviolet-induced DNA damage and double-strand breaks. Two transcript variants encoding the same protein but differing in the 5' UTR, have been found for CBX3.

Molecular Weight: 24kD

Gene ID: 11335

UniProt: Q13185