Collagen Type I antibody

Catalog No. ABIN7228016

The Rabbit Polyclonal anti-Collagen Type I antibody is suitable to detect Collagen Type I in samples from Human, Mouse and Rat. It has been validated for WB and ELISA.

Quick Overview for Collagen Type I antibody (ABIN7228016)

Target: Collagen Type I (COL1) (Collagen, Type I (COL1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Collagen Type I antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Collagen Type I Antibody

Purpose: Rabbit Anti-Collagen I Polyclonal Antibody

Specificity: This antibody detects endogenous levels of Collagen I.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from part region of human Collagen I protein

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,ELISA 1:10000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Collagen Type I

Target: Collagen Type I (COL1) (Collagen, Type I (COL1))

Alternative Name: Collagen I

Background: Collagen alpha-2(I) chain, Alpha-2 type I collagenCOL1A2 encodes the pro-alpha2 chain of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in COL1A2 are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIB, recessive Ehlers-Danlos syndrome Classical type, idiopathic osteoporosis, and atypical Marfan syndrome. Symptoms associated with mutations in COL1A2 , however, tend to be less severe than mutations in the gene for the alpha1 chain of type I collagen (COL1A1) reflecting the different role of alpha2 chains in matrix integrity. Three transcripts, resulting from the use of alternate polyadenylation signals, have been identified for COL1A2.

Molecular Weight: 115kD

Gene ID: 1278

UniProt: P08123