Cortactin antibody (pTyr421)

Catalog No. ABIN7220266

This Rabbit Polyclonal antibody specifically detects Cortactin in WB, IHC, ELISA and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Cortactin antibody (pTyr421) (ABIN7220266)

Target: Cortactin (CTTN)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cortactin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-Cortactin Antibody

Binding Specificity: pTyr421

Purpose: Rabbit Anti-Cortactin (phospho Tyr421) Polyclonal Antibody

Specificity: Phospho-Cortactin (Y421) Polyclonal Antibody detects endogenous levels of Cortactin protein only when phosphorylated at Y421.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human Cortactin Phospho-Tyr421

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:20000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Cortactin

Target: Cortactin (CTTN)

Alternative Name: Cortactin

Background: CTTN, EMS1, Src substrate cortactin, Amplaxin, Oncogene EMS1CTTN is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The cortactin is localized in the cytoplasm and in areas of the cell-substratum contacts. CTTN has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Three splice variants that encode different isoforms have been identified for this gene.

Molecular Weight: 80kD

Gene ID: 2017

UniProt: Q14247

Pathways: MAPK Signaling