Cullin 4B antibody (Internal Region)

Catalog No. ABIN7217916

The Rabbit Polyclonal anti-Cullin 4B antibody (ABIN7217916) specifically detects Cullin 4B in WB, ELISA, IHC and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Cullin 4B antibody (Internal Region) (ABIN7217916)

Target: Cullin 4B (CUL4B)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cullin 4B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-Cullin 4B Antibody

Binding Specificity: Internal Region

Purpose: Rabbit Anti-CUL-4B Polyclonal Antibody

Specificity: CUL-4B Polyclonal Antibody detects endogenous levels of CUL-4B protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human CUL-4B

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC: 1:100-300,ELISA 1:20000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Cullin 4B

Target: Cullin 4B (CUL4B)

Alternative Name: CUL-4B

Background: CUL4B, KIAA0695, Cullin-4B, CUL-4BCUL4B is a member of the cullin family. The cullin 4B forms a complex that functions as an E3 ubiquitin ligase and catalyzes the polyubiquitination of specific protein substrates in the cell. The protein interacts with a ring finger protein, and is required for the proteolysis of several regulators of DNA replication including chromatin licensing and DNA replication factor 1 and cyclin E. Multiple transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 110kD

Gene ID: 8450

UniProt: Q13620