Cyclin B1 antibody

Catalog No. ABIN7225716

This Rabbit Polyclonal antibody specifically detects Cyclin B1 in WB, IHC and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Cyclin B1 antibody (ABIN7225716)

Target: Cyclin B1 (CCNB1)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cyclin B1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-Cyclin B1 Antibody

Purpose: Rabbit Anti-Cyclin B1 Polyclonal Antibody

Specificity: Cyclin B1 Polyclonal Antibody detects endogenous levels of Cyclin B1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human Cyclin B1 around the non-phosphorylation site of S147

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:50-1:200,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Cyclin B1

Target: Cyclin B1 (CCNB1)

Alternative Name: Cyclin B1

Background: CCNB1, CCNB, G2/mitotic-specific cyclin-B1The protein encoded by CCNB1 (cyclin B1) is a regulatory protein involved in mitosis. The gene product complexes with p34 (cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.

Molecular Weight: 65kD

Gene ID: 891

UniProt: P14635

Pathways: Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase