CDC2 / CDK2 antibody (pThr160)

Catalog No. ABIN7213224

This Rabbit Polyclonal antibody specifically detects CDC2 / CDK2 in ELISA, WB, IHC and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for CDC2 / CDK2 antibody (pThr160) (ABIN7213224)

Target: CDC2 / CDK2 (CDC2) (Cyclin-Dependent Kinase A-1 (CDC2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDC2 / CDK2 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-CDC2 / CDK2 Antibody

Binding Specificity: pThr160

Purpose: Rabbit Anti-Cdk2/Cdc2 (phospho Thr160) Polyclonal Antibody

Specificity: Phospho-Cdk2/Cdc2 (T160) Polyclonal Antibody detects endogenous levels of Cdk2/Cdc2 protein only when phosphorylated at T160.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human Cdk2/Cdc2 Phospho-Thr160

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:20000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for CDC2 / CDK2

Target: CDC2 / CDK2 (CDC2) (Cyclin-Dependent Kinase A-1 (CDC2))

Alternative Name: Cdk2/Cdc2

Background: CDK2, CDKN2, Cyclin-dependent kinase 2, Cell division protein kinase 2, p33 protein kinaseCDK2 encodes a member of a family of serine/threonine protein kinases that participate in cell cycle regulation. Cyclin dependent kinase 2 is the catalytic subunit of the cyclin-dependent protein kinase complex, which regulates progression through the cell cycle. Activity of this protein is especially critical during the G1 to S phase transition. This protein associates with and regulated by other subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A), and p27Kip1 (CDKN1B). Alternative splicing results in multiple transcript variants.

Molecular Weight: 34kD

Gene ID: 1017

UniProt: P24941