POLI antibody (AA 610-690)

Catalog No. ABIN7219268

This Rabbit Polyclonal antibody specifically detects POLI in WB and ELISA. It exhibits reactivity toward Human and Mouse.

Quick Overview for POLI antibody (AA 610-690) (ABIN7219268)

Target: POLI (Polymerase (DNA Directed) iota (POLI))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLI antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-POLI Antibody

Binding Specificity: AA 610-690

Purpose: Rabbit Anti-DNA pol ι Polyclonal Antibody

Specificity: DNA pol ι Polyclonal Antibody detects endogenous levels of DNA pol ι protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the C-terminal region of human DNA pol ι at AA range: 610-690

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,ELISA 1:40000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for POLI

Target: POLI (Polymerase (DNA Directed) iota (POLI))

Alternative Name: DNA pol ι

Background: POLI, RAD30B, DNA polymerase iota, Eta2, RAD30 homolog BPOLI (Polymerase (DNA) Iota) is a Protein Coding gene. Among its related pathways are DNA Double-Strand Break Repair and Fanconi anemia pathway. GO annotations related to this gene include damaged DNA binding and DNA-directed DNA polymerase activity. An important paralog of this gene is POLH. rror-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunogobulin genes. Forms a Schiff base with 5-deoxyribose phosphate at abasic sites, but may not have lyase activity.

Molecular Weight: 85kD

Gene ID: 11201

UniProt: Q9UNA4

Pathways: DNA Damage Repair