E2F2 antibody (AA 190-270)

Catalog No. ABIN7214646

The Rabbit Polyclonal anti-E2F2 antibody is suitable to detect E2F2 in samples from Human and Mouse. It has been validated for WB, ELISA and IHC (p).

Quick Overview for E2F2 antibody (AA 190-270) (ABIN7214646)

Target: E2F2 (E2F Transcription Factor 2 (E2F2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This E2F2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-E2F2 Antibody

Binding Specificity: AA 190-270

Purpose: E2F-2 Polyclonal Antibody

Specificity: E2F-2 Polyclonal Antibody detects endogenous levels of E2F-2 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of human E2F-2 at AA range: 190-270

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:40000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for E2F2

Target: E2F2 (E2F Transcription Factor 2 (E2F2))

Alternative Name: E2F-2

Background: Rabbit Anti-E2F-2 Polyclonal Antibody,E2F2, Transcription factor E2F2, E2F-2,E2F transcription factor 2 encoded by E2F2 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F1 and E2F3, have an additional cyclin binding domain. This protein binds specifically to retinoblastoma protein pRB in a cell-cycle dependent manner, and it exhibits overall 46 % amino acid identity to E2F1.,Transcription factor E2F2

Molecular Weight: observerd band 48kDa

Gene ID: 1870

UniProt: Q14209

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication