E2F2 antibody (AA 190-270)

Catalog No. ABIN7214646

The Rabbit Polyclonal anti-E2F2 antibody is suitable to detect E2F2 in samples from Human and Mouse. It has been validated for WB, ELISA, IHC and IF.

Quick Overview for E2F2 antibody (AA 190-270) (ABIN7214646)

Target: E2F2 (E2F Transcription Factor 2 (E2F2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This E2F2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-E2F2 Antibody

Binding Specificity: AA 190-270

Purpose: Rabbit Anti-E2F-2 Polyclonal Antibody

Specificity: E2F-2 Polyclonal Antibody detects endogenous levels of E2F-2 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human E2F-2 at AA range: 190-270

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:40000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for E2F2

Target: E2F2 (E2F Transcription Factor 2 (E2F2))

Alternative Name: E2F-2

Background: E2F2, Transcription factor E2F2, E2F-2E2F transcription factor 2 encoded by E2F2 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F1 and E2F3, have an additional cyclin binding domain. This protein binds specifically to retinoblastoma protein pRB in a cell-cycle dependent manner, and it exhibits overall 46 % amino acid identity to E2F1.

Molecular Weight: 48kD

Gene ID: 1870

UniProt: Q14209

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication