NEIL2 antibody

Catalog No. ABIN7229952

The Rabbit Polyclonal anti-NEIL2 antibody is suitable to detect NEIL2 in samples from Human and Mouse. It has been validated for WB and ELISA.

Quick Overview for NEIL2 antibody (ABIN7229952)

Target: NEIL2 (Endonuclease 8-like 2 (NEIL2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NEIL2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-NEIL2 Antibody

Purpose: Rabbit Anti-NEIL2 Polyclonal Antibody

Specificity: The antibody detects endogenous levels of NEIL2 protein

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from part region of human NEIL2 protein

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,ELISA 1:5000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 %BSA

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for NEIL2

Target: NEIL2 (Endonuclease 8-like 2 (NEIL2))

Alternative Name: NEIL2

Background: Endonuclease 8-like 2, DNA glycosylase/AP lyase Neil2, DNA-(apurinic or apyrimidinic site) lyase Neil2, Endonuclease VIII-like 2, Nei homolog 2, NEH2, Nei-like protein 2NEIL2 encodes a member of the Fpg/Nei family of DNA glycosylases. These glycosylases initiate the first step in base excision repair by cleaving oxidatively damaged bases and introducing a DNA strand break via their abasic site lyase activity. This enzyme is primarily associated with DNA repair during transcription and acts prefentially on cytosine-derived lesions, particularly 5-hydroxyuracil and 5-hydroxycytosine. It contains an N-terminal catalytic domain, a hinge region, and a C-terminal DNA-binding domain with helix-two-turn-helix and zinc finger motifs. This enzyme interacts with the X-ray cross complementing factor 1 scaffold protein as part of a multi-protein DNA repair complex. A pseudogene of this gene has been identified.

Molecular Weight: 36kD

Gene ID: 252969

UniProt: Q969S2

Pathways: DNA Damage Repair