Endothelin 1 antibody (Internal Region)

Catalog No. ABIN7217844

The Rabbit Polyclonal anti-Endothelin 1 antibody (ABIN7217844) specifically detects Endothelin 1 in WB, IHC, ELISA and IF. The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for Endothelin 1 antibody (Internal Region) (ABIN7217844)

Target: Endothelin 1 (EDN1)

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Endothelin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-Endothelin 1 Antibody

Binding Specificity: Internal Region

Purpose: Rabbit Anti-ET-1 Polyclonal Antibody

Specificity: ET-1 Polyclonal Antibody detects endogenous levels of ET-1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human ET-1

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC: 100-300,WB 1:500-1:2000,ELISA 1:20000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Endothelin 1

Target: Endothelin 1 (EDN1)

Alternative Name: ET-1

Background: EDN1, Endothelin-1, Preproendothelin-1, PPET1EDN1 encodes a preproprotein that is proteolytically processed to generate a secreted peptide that belongs to the endothelin/sarafotoxin family. This peptide is a potent vasoconstrictor and its cognate receptors are therapeutic targets in the treatment of pulmonary arterial hypertension. Aberrant expression of this gene may promote tumorigenesis. Alternative splicing results in multiple transcript variants.

Molecular Weight: 25kD

Gene ID: 1906

UniProt: P05305

Pathways: Hormone Transport, Negative Regulation of Hormone Secretion, Regulation of Systemic Arterial Blood Pressure by Hormones, cAMP Metabolic Process, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, Regulation of Cell Size