EEF2K antibody (pSer366)

Catalog No. ABIN7213234

This Rabbit Polyclonal antibody specifically detects EEF2K in WB, ELISA, IHC and IF. It exhibits reactivity toward Human and Rat.

Quick Overview for EEF2K antibody (pSer366) (ABIN7213234)

Target: EEF2K (Eukaryotic Elongation Factor-2 Kinase (EEF2K))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EEF2K antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-EEF2K Antibody

Binding Specificity: pSer366

Purpose: Rabbit Anti-eEF2K (phospho Ser366) Polyclonal Antibody

Specificity: Phospho-eEF2K (S366) Polyclonal Antibody detects endogenous levels of eEF2K protein only when phosphorylated at S366.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human eEF2K Phospho-Ser366

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:40000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for EEF2K

Target: EEF2K (Eukaryotic Elongation Factor-2 Kinase (EEF2K))

Alternative Name: eEF2K

Background: EEF2K, Eukaryotic elongation factor 2 kinase, eEF-2 kinase, eEF-2K, Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinaseEEF2K encodes a highly conserved protein kinase (eukaryotic elongation factor 2 kinase) in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. This kinase is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2) and thus inhibits the EEF2 function. The activity of eukaryotic elongation factor 2 kinase is increased in many cancers and may be a valid target for anti-cancer treatment.

Molecular Weight: 105kD

Gene ID: 29904

UniProt: O00418

Pathways: AMPK Signaling