EEF2K antibody

Catalog No. ABIN7214676

The Rabbit Polyclonal anti-EEF2K antibody is suitable to detect EEF2K in samples from Human and Rat. It has been validated for WB, ELISA, IHC and IF.

Quick Overview for EEF2K antibody (ABIN7214676)

Target: EEF2K (Eukaryotic Elongation Factor-2 Kinase (EEF2K))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EEF2K antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-EEF2K Antibody

Purpose: Rabbit Anti-eEF2K Polyclonal Antibody

Specificity: EEF2K Polyclonal Antibody detects endogenous levels of eEF2K protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human eEF2K around the non-phosphorylation site of S366

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:20000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for EEF2K

Target: EEF2K (Eukaryotic Elongation Factor-2 Kinase (EEF2K))

Alternative Name: eEF2K

Background: EEF2K, Eukaryotic elongation factor 2 kinase, eEF-2 kinase, eEF-2K, Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinaseEEF2K encodes a highly conserved protein kinase in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. Eukaryotic elongation factor 2 kinase is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2) and thus inhibits the EEF2 function. The activity of this kinase is increased in many cancers and may be a valid target for anti-cancer treatment.

Molecular Weight: 105kD

Gene ID: 29904

UniProt: O00418

Pathways: AMPK Signaling