Factor VII antibody (Cleaved-Arg212)

Catalog No. ABIN7212512

The Rabbit Polyclonal anti-Factor VII antibody (ABIN7212512) specifically detects Factor VII in WB, IHC, ELISA and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Factor VII antibody (Cleaved-Arg212) (ABIN7212512)

Target: Factor VII (F7) (Coagulation Factor VII (F7))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Factor VII antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-Factor VII Antibody

Binding Specificity: AA 140-220, Cleaved-Arg212

Purpose: Rabbit Anti-Cleaved-Factor VII LC (R212) Polyclonal Antibody

Specificity: Cleaved-Factor VII LC (R212) Polyclonal Antibody detects endogenous levels of fragment of activated Factor VII LC protein resulting from cleavage adjacent to R212.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human Factor VII LC at AA range: 140-220

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:20000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Factor VII

Target: Factor VII (F7) (Coagulation Factor VII (F7))

Alternative Name: Factor VII LC

Background: F7, Coagulation factor VII, Proconvertin, Serum prothrombin conversion accelerator, SPCA, Eptacog alfaF7 encodes coagulation factor VII which is a vitamin K-dependent factor essential for hemostasis. This factor circulates in the blood in a zymogen form, and is converted to an active form by either factor IXa, factor Xa, factor XIIa, or thrombin by minor proteolysis. Upon activation of the factor VII, a heavy chain containing a catalytic domain and a light chain containing 2 EGF-like domains are generated, and two chains are held together by a disulfide bond. In the presence of factor III and calcium ions, the activated factor then further activates the coagulation cascade by converting factor IX to factor IXa and/or factor X to factor Xa. Defects in F7 can cause coagulopathy. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing to generate mature polypeptides.

Molecular Weight: 17kD

Gene ID: 2155

UniProt: P08709

Pathways: Response to Growth Hormone Stimulus, Platelet-derived growth Factor Receptor Signaling