FOXO1 antibody (pThr24, pThr32)

Catalog No. ABIN7220640

The Rabbit Polyclonal anti-FOXO1 antibody is suitable to detect FOXO1 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA and IHC (p).

Quick Overview for FOXO1 antibody (pThr24, pThr32) (ABIN7220640)

Target: FOXO1 (Forkhead Box O1 (FOXO1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FOXO1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-FOXO1 Antibody

Binding Specificity: pThr24, pThr32

Purpose: FoxO1/3/4 (phospho Thr24/32) Polyclonal Antibody

Specificity: Phospho-FoxO1/3/4 (T24/32) Polyclonal Antibody detects endogenous levels of FoxO1/3/4 protein only when phosphorylated at T24/32.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human FoxO1/3/4 Phospho-Thr24/32

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:5000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for FOXO1

Target: FOXO1 (Forkhead Box O1 (FOXO1))

Alternative Name: FoxO1/3/4

Background: Rabbit Anti-FoxO1/3/4 (phospho Thr24/32) Polyclonal Antibody,FOXO1, FKHR, FOXO1A, Forkhead box protein O1, Forkhead box protein O1A, Forkhead in rhabdomyosarcoma, FOXO3, FKHRL1, FOXO3A, Forkhead box protein O3, AF6q21 protein, Forkhead in rhabdomyosarcoma-like 1, FOXO4, AFX, AFX1, MLLT7, Forkhead box,FOXO1 belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined, however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma.,Forkhead box protein O1

Molecular Weight: observerd band 78kDa

Gene ID: 2308, 4303

Pathways: PI3K-Akt Signaling, Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis, Chromatin Binding, Regulation of Carbohydrate Metabolic Process, CXCR4-mediated Signaling Events, BCR Signaling