FHIT antibody (AA 60-140)

Catalog No. ABIN7214896

This Rabbit Polyclonal antibody specifically detects FHIT in ELISA, WB, IF and IHC (p). It exhibits reactivity toward Human.

Quick Overview for FHIT antibody (AA 60-140) (ABIN7214896)

Target: FHIT (Fragile Histidine Triad (FHIT))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FHIT antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-FHIT Antibody

Binding Specificity: AA 60-140

Purpose: Fhit Polyclonal Antibody

Specificity: Fhit Polyclonal Antibody detects endogenous levels of Fhit protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of human Fhit at AA range: 60-140

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:10000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for FHIT

Target: FHIT (Fragile Histidine Triad (FHIT))

Alternative Name: Fhit

Background: Rabbit Anti-Fhit Polyclonal Antibody,FHIT, Bis(5'-adenosyl)-triphosphatase, AP3A hydrolase, AP3Aase, Diadenosine 5', 5'''-P1,P3-triphosphate hydrolase, Dinucleosidetriphosphatase, Fragile histidine triad protein,FHIT, a member of the histidine triad gene family, encodes a diadenosine 5',5'''-P1,P3-triphosphate hydrolase involved in purine metabolism. FHIT encompasses the common fragile site FRA3B on chromosome 3, where carcinogen-induced damage can lead to translocations and aberrant transcripts of FHIT. In fact, aberrant transcripts from FHIT have been found in about half of all esophageal, stomach, and colon carcinomas. Alternatively spliced transcript variants have been found for FHIT.,Bis(5'-adenosyl)-triphosphatase

Molecular Weight: observerd band 16kDa

Gene ID: 2272

UniProt: P49789