FHIT antibody (pTyr114)

Catalog No. ABIN7220538

The Rabbit Polyclonal anti-FHIT antibody is suitable to detect FHIT in samples from Human, Mouse and Rat. It has been validated for ELISA, IHC and IF.

Quick Overview for FHIT antibody (pTyr114) (ABIN7220538)

Target: FHIT (Fragile Histidine Triad (FHIT))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FHIT antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-FHIT Antibody

Binding Specificity: pTyr114

Purpose: Rabbit Anti-Fhit (phospho Tyr114) Polyclonal Antibody

Specificity: Phospho-Fhit (Y114) Polyclonal Antibody detects endogenous levels of Fhit protein only when phosphorylated at Y114.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human Fhit Phospho-Tyr114

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC 1:100-1:300,ELISA 1:40000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for FHIT

Target: FHIT (Fragile Histidine Triad (FHIT))

Alternative Name: Fhit

Background: FHIT, Bis(5'-adenosyl)-triphosphatase, AP3A hydrolase, AP3Aase, Diadenosine 5', 5'''-P1,P3-triphosphate hydrolase, Dinucleosidetriphosphatase, Fragile histidine triad proteinFHIT, a member of the histidine triad gene family, encodes a diadenosine 5',5'''-P1,P3-triphosphate hydrolase involved in purine metabolism. The gene encompasses the common fragile site FRA3B on chromosome 3, where carcinogen-induced damage can lead to translocations and aberrant transcripts of this gene. In fact, aberrant transcripts from this gene have been found in about half of all esophageal, stomach, and colon carcinomas. Alternatively spliced transcript variants have been found for this gene.

Gene ID: 2272

UniProt: P49789