GFM2 antibody (AA 410-490)

Catalog No. ABIN7225406

This Rabbit Polyclonal antibody specifically detects GFM2 in WB, ELISA, IHC and IF. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for GFM2 antibody (AA 410-490) (ABIN7225406)

Target: GFM2 (G Elongation Factor, Mitochondrial 2 (GFM2))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GFM2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-GFM2 Antibody

Binding Specificity: AA 410-490

Purpose: Rabbit Anti-EF-G2 Polyclonal Antibody

Specificity: EF-G2 Polyclonal Antibody detects endogenous levels of EF-G2 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human EF-G2 at AA range: 410-490

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:20000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for GFM2

Target: GFM2 (G Elongation Factor, Mitochondrial 2 (GFM2))

Alternative Name: EF-G2

Background: GFM2, EFG2, MSTP027, Ribosome-releasing factor 2, mitochondrial, RRF2mt, Elongation factor G 2, mitochondrial, EF-G2mt, mEF-G 2, Elongation factor G2, hEFG2Eukaryotes contain two protein translational systems, one in the cytoplasm and one in the mitochondria. Mitochondrial translation is crucial for maintaining mitochondrial function and mutations in this system lead to a breakdown in the respiratory chain-oxidative phosphorylation system and to impaired maintenance of mitochondrial DNA. GFM2 (G elongation factor mitochondrial 2) encodes one of the mitochondrial translation elongation factors, which is a GTPase that plays a role at the termination of mitochondrial translation by mediating the disassembly of ribosomes from messenger RNA. Its role in the regulation of normal mitochondrial function and in disease states attributed to mitochondrial dysfunction is not known. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Molecular Weight: 87kD

Gene ID: 84340

UniProt: Q969S9

Pathways: Ribonucleoprotein Complex Subunit Organization