GDNF antibody (AA 100-180)

Catalog No. ABIN7228642

This Rabbit Polyclonal antibody specifically detects GDNF in WB and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for GDNF antibody (AA 100-180) (ABIN7228642)

Target: GDNF (Glial Cell Line Derived Neurotrophic Factor (GDNF))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GDNF antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-GDNF Antibody

Binding Specificity: AA 100-180

Purpose: Rabbit Anti-GDNF Polyclonal Antibody

Specificity: The antibody detects endogenous levels of GDNF protein

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from part region of human GDNF protein at AA range: 100-180

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,ELISA 1:5000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 %BSA

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for GDNF

Target: GDNF (Glial Cell Line Derived Neurotrophic Factor (GDNF))

Alternative Name: GDNF

Background: Glial cell line-derived neurotrophic factor, hGDNF, Astrocyte-derived trophic factor, ATFGDNF encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. The recombinant form of this protein, a highly conserved neurotrophic factor, was shown to promote the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosis of motor neurons induced by axotomy. This protein is a ligand for the product of the RET (rearranged during transfection) protooncogene. Mutations in GDNF may be associated with Hirschsprung disease and Tourette syndrome. GDNF encodes multiple protein isoforms that may undergo similar proteolytic processing.

Molecular Weight: 23kD

Gene ID: 2668

UniProt: P39905

Pathways: RTK Signaling, Synaptic Membrane, Tube Formation, Autophagy, Smooth Muscle Cell Migration