GSK3 alpha antibody (pSer21)

Catalog No. ABIN7212976

The Rabbit Polyclonal anti-GSK3 alpha antibody has been validated for WB, ELISA, IP and IHC (p). It is suitable to detect GSK3 alpha in samples from Human, Mouse and Rat.

Quick Overview for GSK3 alpha antibody (pSer21) (ABIN7212976)

Target: GSK3 alpha (GSK3a) (Glycogen Synthase Kinase 3 alpha (GSK3a))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GSK3 alpha antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-GSK3 alpha Antibody

Binding Specificity: pSer21

Purpose: GSK3α (phospho Ser21) Polyclonal Antibody

Specificity: Phospho-GSK3α (S21) Polyclonal Antibody detects endogenous levels of GSK3α protein only when phosphorylated at S21.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human GSK3alpha Phospho-Ser21

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IP (2-5 μg/mg lysate), ELISA (1:20000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for GSK3 alpha

Target: GSK3 alpha (GSK3a) (Glycogen Synthase Kinase 3 alpha (GSK3a))

Alternative Name: GSK3alpha

Background: Rabbit Anti-GSK3α (phospho Ser21) Polyclonal Antibody,GSK3A, Glycogen synthase kinase-3 alpha, GSK-3 alpha, Serine/threonine-protein kinase GSK3A,GSK3A encodes a multifunctional Ser/Thr protein kinase (glycogen synthase kinase 3 alpha) that is implicated in the control of several regulatory proteins including glycogen synthase, and transcription factors, such as JUN. It also plays a role in the WNT and PI3K signaling pathways, as well as regulates the production of beta-amyloid peptides associated with Alzheimer's disease.,Glycogen synthase kinase-3 alpha

Molecular Weight: observerd band 55kDa

Gene ID: 2931

UniProt: P49840

Pathways: PI3K-Akt Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, BCR Signaling, Warburg Effect