IDE antibody

Catalog No. ABIN7211960

This Mouse Monoclonal antibody specifically detects IDE in WB, IHC and IF. It exhibits reactivity toward Human and Hamster.

Quick Overview for IDE antibody (ABIN7211960)

Target: IDE (Insulin-Degrading Enzyme (IDE))

Reactivity: Human, Hamster

Host: Mouse

Clonality: Monoclonal

Conjugate: This IDE antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-IDE Antibody

Purpose: Mouse Anti-IDE Monoclonal Antibody

Specificity: The antibody detects endogenous IDE proteins.

Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Immunogen: Synthetic Peptide

Isotype: IgG1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:1000,IF 1:200,IHC 1:50-300

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4, containing 0.5 % BSA, 0.02 % sodium azide as Preservative and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for IDE

Target: IDE (Insulin-Degrading Enzyme (IDE))

Alternative Name: IDE

Background: IDE, Insulin-degrading enzyme, Abeta-degrading protease, Insulin protease, Insulinase, InsulysinIDE encodes a zinc metallopeptidase that degrades intracellular insulin, and thereby terminates insulins activity, as well as participating in intercellular peptide signalling by degrading diverse peptides such as glucagon, amylin, bradykinin, and kallidin. The preferential affinity of insulin degrading enzyme for insulin results in insulin-mediated inhibition of the degradation of other peptides such as beta-amyloid. Deficiencies in this protein's function are associated with Alzheimer's disease and type 2 diabetes mellitus but mutations in IDE have not been shown to be causitive for these diseases. Insulin degrading enzyme localizes primarily to the cytoplasm but in some cell types localizes to the extracellular space, cell membrane, peroxisome, and mitochondrion. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described but have not been experimentally verified.

Molecular Weight: 118kD

Gene ID: 3416

UniProt: P14735

Pathways: SARS-CoV-2 Protein Interactome