IGF1R antibody (pTyr1161)

Catalog No. ABIN7213316

The Rabbit Polyclonal anti-IGF1R antibody (ABIN7213316) specifically detects IGF1R in WB, IHC, ELISA and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for IGF1R antibody (pTyr1161) (ABIN7213316)

Target: IGF1R (Insulin-Like Growth Factor 1 Receptor (IGF1R))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IGF1R antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-IGF1R Antibody

Binding Specificity: pTyr1161

Purpose: Rabbit Anti-IGF-IR (phospho Tyr1161) Polyclonal Antibody

Specificity: Phospho-IGF-IR (Y1161) Polyclonal Antibody detects endogenous levels of IGF-IR protein only when phosphorylated at Y1161.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human IGF-IR Phospho-Tyr1161

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:40000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for IGF1R

Target: IGF1R (Insulin-Like Growth Factor 1 Receptor (IGF1R))

Alternative Name: IGF-IR

Background: IGF1R, Insulin-like growth factor 1 receptor, Insulin-like growth factor I receptor, IGF-I receptor, CD antigen CD221, INSR, Insulin receptor, IR, CD antigen CD220Insulin like growth factor 1 receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. Alternatively spliced transcript variants encoding distinct isoforms have been found for IGF1R.

Molecular Weight: pro:155kD,recetor beta:95kD

Gene ID: 3480

UniProt: P08069, P06213

Pathways: RTK Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Autophagy