Integrin beta 2 antibody (pThr758)

Catalog No. ABIN7221898

The Rabbit Polyclonal anti-Integrin beta 2 antibody has been validated for ELISA and IF. It is suitable to detect Integrin beta 2 in samples from Human, Mouse and Rat.

Quick Overview for Integrin beta 2 antibody (pThr758) (ABIN7221898)

Target: Integrin beta 2 (ITGB2)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Integrin beta 2 antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF)

Product Details anti-Integrin beta 2 Antibody

Binding Specificity: pThr758

Purpose: Integrin β2 (phospho Thr758) Polyclonal Antibody

Specificity: Phospho-Integrin β2 (T758) Polyclonal Antibody detects endogenous levels of Integrin β2 protein only when phosphorylated at T758.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human Integrin beta2 Phospho-Thr758

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IF (1:200-1:1000), ELISA (1:40000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for Integrin beta 2

Target: Integrin beta 2 (ITGB2)

Alternative Name: Integrin beta2

Background: Rabbit Anti-Integrin β2 (phospho Thr758) Polyclonal Antibody,ITGB2, CD18, MFI7, Integrin beta-2, Cell surface adhesion glycoproteins LFA-1/CR3/p150, 95 subunit beta, Complement receptor C3 subunit beta, CD antigen CD18,ITGB2 encodes an integrin beta chain, which combines with multiple different alpha chains to form different integrin heterodimers. Integrins are integral cell-surface proteins that participate in cell adhesion as well as cell-surface mediated signalling. The encoded protein plays an important role in immune response and defects in this gene cause leukocyte adhesion deficiency. Alternative splicing results in multiple transcript variants.,Integrin beta-2

Molecular Weight: observerd band 170kDa

Gene ID: 3689

UniProt: P05107

Pathways: NF-kappaB Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation