LIG1 antibody (AA 80-160)

Catalog No. ABIN7214586

This Rabbit Polyclonal antibody specifically detects LIG1 in WB, IHC, ELISA and IF. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for LIG1 antibody (AA 80-160) (ABIN7214586)

Target: LIG1 (Ligase I, DNA, ATP-Dependent (LIG1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LIG1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-LIG1 Antibody

Binding Specificity: AA 80-160

Purpose: Rabbit Anti-DNA Ligase I Polyclonal Antibody

Specificity: DNA Ligase I Polyclonal Antibody detects endogenous levels of DNA Ligase I protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the N-terminal region of human DNA Ligase I at AA range: 80-160

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:20000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for LIG1

Target: LIG1 (Ligase I, DNA, ATP-Dependent (LIG1))

Alternative Name: DNA Ligase I

Background: LIG1, DNA ligase 1, DNA ligase I, Polydeoxyribonucleotide synthase [ATP] 1LIG1 encodes a member of the ATP-dependent DNA ligase protein family. DNA ligase 1 functions in DNA replication, recombination, and the base excision repair process. Mutations in this gene that lead to DNA ligase I deficiency result in immunodeficiency and increased sensitivity to DNA-damaging agents. Disruption of LIG1 may also be associated with a variety of cancers. Alternative splicing results in multiple transcript variants.

Molecular Weight: 133kD

Gene ID: 3978

UniProt: P18858

Pathways: Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA