MDC1 antibody (pSer513)

Catalog No. ABIN7226058

This Rabbit Polyclonal antibody specifically detects MDC1 in IHC, ELISA and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for MDC1 antibody (pSer513) (ABIN7226058)

Target: MDC1 (Mdc1 (MDC1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MDC1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-MDC1 Antibody

Binding Specificity: pSer513

Purpose: Rabbit Anti-MDC1 (phospho Ser513) Polyclonal Antibody

Specificity: Phospho-MDC1 (S513) Polyclonal Antibody detects endogenous levels of MDC1 protein only when phosphorylated at S513.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human MDC1 Phospho-Ser513

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC 1:100-1:300,ELISA 1:5000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for MDC1

Target: MDC1 (Mdc1 (MDC1))

Alternative Name: MDC1

Background: MDC1, KIAA0170, NFBD1, Mediator of DNA damage checkpoint protein 1, Nuclear factor with BRCT domains 1The protein encoded by MDC1 (mediator of DNA damage checkpoint 1) contains an N-terminal forkhead domain, two BRCA1 C-terminal (BRCT) motifs and a central domain with 13 repetitions of an approximately 41-amino acid sequence. The encoded protein is required to activate the intra-S phase and G2/M phase cell cycle checkpoints in response to DNA damage. This nuclear protein interacts with phosphorylated histone H2AX near sites of DNA double-strand breaks through its BRCT motifs, and facilitates recruitment of the ATM kinase and meiotic recombination 11 protein complex to DNA damage foci.

Gene ID: 9656

UniProt: Q14676