MED24 antibody (AA 770-850)

Catalog No. ABIN7217378

The Rabbit Polyclonal anti-MED24 antibody (ABIN7217378) specifically detects MED24 in ELISA, WB, IF and IHC. The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for MED24 antibody (AA 770-850) (ABIN7217378)

Target: MED24 (Mediator Complex Subunit 24 (MED24))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED24 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)

Product Details anti-MED24 Antibody

Binding Specificity: AA 770-850

Purpose: Rabbit Anti-TRAP100 Polyclonal Antibody

Specificity: TRAP100 Polyclonal Antibody detects endogenous levels of TRAP100 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the C-terminal region of human TRAP100 at AA range: 770-850

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:20000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for MED24

Target: MED24 (Mediator Complex Subunit 24 (MED24))

Alternative Name: TRAP100

Background: MED24, ARC100, CRSP4, DRIP100, KIAA0130, THRAP4, TRAP100, Mediator of RNA polymerase II transcription subunit 24, Activator-recruited cofactor 100 kDa component, ARC100, Cofactor required for Sp1 transcriptional activation subunit 4, CRSP cMED24 encodes a component of the mediator complex (also known as TRAP, SMCC, DRIP, or ARC), a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, possibly by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. Multiple transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 110kD

Gene ID: 9862

UniProt: O75448

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha, Positive Regulation of Endopeptidase Activity