MED8 antibody (AA 70-150)

Catalog No. ABIN7219308

The Rabbit Polyclonal anti-MED8 antibody is suitable to detect MED8 in samples from Human, Mouse and Rat. It has been validated for WB and ELISA.

Quick Overview for MED8 antibody (AA 70-150) (ABIN7219308)

Target: MED8 (Mediator Complex Subunit 8 (MED8))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED8 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-MED8 Antibody

Binding Specificity: AA 70-150

Purpose: Med8 Polyclonal Antibody

Specificity: Med8 Polyclonal Antibody detects endogenous levels of Med8 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of human Med8 at AA range: 70-150

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:5000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for MED8

Target: MED8 (Mediator Complex Subunit 8 (MED8))

Alternative Name: Med8

Background: Rabbit Anti-Med8 Polyclonal Antibody,MED8, Mediator of RNA polymerase II transcription subunit 8, Activator-recruited cofactor 32 kDa component, ARC32, Mediator complex subunit 8,MED8 encodes a protein component of the mediator complex, which aids in transcriptional activation through interaction with RNA polymerase II and gene-specific transcription factors. The mediator complex subunit 8 may also function in ubiquitin ligation and protein degradation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.,Mediator of RNA polymerase II transcription subunit 8

Molecular Weight: observerd band 29kDa

Gene ID: 112950

UniProt: Q96G25

Pathways: Regulation of Lipid Metabolism by PPARalpha