MIB1 antibody (AA 901-950)

Catalog No. ABIN7226942

This Rabbit Polyclonal antibody specifically detects MIB1 in WB, ELISA, IHC and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for MIB1 antibody (AA 901-950) (ABIN7226942)

Target: MIB1 (Mindbomb E3 Ubiquitin Protein Ligase 1 (MIB1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MIB1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-MIB1 Antibody

Binding Specificity: AA 901-950

Purpose: Rabbit Anti-MIB1 Polyclonal Antibody

Specificity: The antibody detects endogenous MIB1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthetic peptide from human protein at AA range: 901-950

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC: 100-300,WB 1:500-2000,ELISA 1:10000-20000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for MIB1

Target: MIB1 (Mindbomb E3 Ubiquitin Protein Ligase 1 (MIB1))

Alternative Name: MIB1

Background: MIB1, DIP1, KIAA1323, ZZANK2MIB1 (mindbomb E3 ubiquitin protein ligase 1) encodes a protein containing multiple ankyrin repeats and RING finger domains that functions as an E3 ubiquitin ligase. The encoded protein positively regulates Notch signaling by ubiquitinating the Notch receptors, thereby facilitating their endocytosis. This protein may also promote the ubiquitination and degradation of death-associated protein kinase 1 (DAPK1).

Molecular Weight: 130kD

Gene ID: 57534

UniProt: Q86YT6

Pathways: SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection