MCM5 antibody (AA 40-120)

Catalog No. ABIN7214114

The Rabbit Polyclonal anti-MCM5 antibody is suitable to detect MCM5 in samples from Human, Mouse and Rat. It has been validated for WB, IHC, IF and ELISA.

Quick Overview for MCM5 antibody (AA 40-120) (ABIN7214114)

Target: MCM5 (Minichromosome Maintenance Complex Component 5 (MCM5))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MCM5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Product Details anti-MCM5 Antibody

Binding Specificity: AA 40-120

Purpose: Rabbit Anti-CDC46 Polyclonal Antibody

Specificity: CDC46 Polyclonal Antibody detects endogenous levels of CDC46 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the N-terminal region of human CDC46 at AA range: 40-120

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:20000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for MCM5

Target: MCM5 (Minichromosome Maintenance Complex Component 5 (MCM5))

Alternative Name: CDC46

Background: MCM5, CDC46, DNA replication licensing factor MCM5, CDC46 homolog, P1-CDC46Minichromosome maintenance complex component 5 encoded by MCM5 is structurally very similar to the CDC46 protein from S. cerevisiae, a protein involved in the initiation of DNA replication. The encoded protein is a member of the MCM family of chromatin-binding proteins and can interact with at least two other members of this family. The encoded protein is upregulated in the transition from the G0 to G1/S phase of the cell cycle and may actively participate in cell cycle regulation.

Molecular Weight: 90kD

Gene ID: 4174

UniProt: P33992

Pathways: DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA