MAP3K4 antibody (AA 1250-1330)

Catalog No. ABIN7222446

The Rabbit Polyclonal anti-MAP3K4 antibody (ABIN7222446) specifically detects MAP3K4 in ELISA and IHC (p). The antibody is reactive with Human and Mouse samples.

Quick Overview for MAP3K4 antibody (AA 1250-1330) (ABIN7222446)

Target: MAP3K4 (Mitogen-Activated Protein Kinase Kinase Kinase 4 (MAP3K4))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAP3K4 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-MAP3K4 Antibody

Binding Specificity: AA 1250-1330

Purpose: MEK Kinase-4 Polyclonal Antibody

Specificity: MEK Kinase-4 Polyclonal Antibody detects endogenous levels of MEK Kinase-4 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the C-terminal region of human MEK Kinase-4 at AA range: 1250-1330

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC-P (1:100-1:300), ELISA (1:5000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for MAP3K4

Target: MAP3K4 (Mitogen-Activated Protein Kinase Kinase Kinase 4 (MAP3K4))

Alternative Name: MEK Kinase-4

Background: Rabbit Anti-MEK Kinase-4 Polyclonal Antibody,MAP3K4, KIAA0213, MAPKKK4, MEKK4, MTK1, Mitogen-activated protein kinase kinase kinase 4, MAP three kinase 1, MAPK/ERK kinase kinase 4, MEK kinase 4, MEKK 4,The central core of each mitogen-activated protein kinase (MAPK) pathway is a conserved cascade of 3 protein kinases: an activated MAPK kinase kinase (MAPKKK) phosphorylates and activates a specific MAPK kinase (MAPKK), which then activates a specific MAPK. While the ERK MAPKs are activated by mitogenic stimulation, the CSBP2 and JNK MAPKs are activated by environmental stresses such as osmotic shock, UV irradiation, wound stress, and inflammatory factors. This gene encodes a MAPKKK, the MEKK4 protein, also called MTK1. This protein contains a protein kinase catalytic domain at the C terminus. The N-terminal nonkinase domain may contain a regulatory domain. Expression of MEKK4 in mammalian cells activated the CSBP2 and JNK MAPK pathways, but not the ERK pathway. In vitro kinase studies indicated that recombinant MEKK4 can specifically phosphorylate and activate PRKMK6 and SERK1, MAPKKs that activate CSBP2 and JNK, respectively but cannot phosphorylate PRKMK1, an MAPKK that activates ERKs. MEKK4 is a major mediator of environmental stresses that activate the CSBP2 MAPK pathway, and a minor mediator of the JNK pathway. Several alternatively spliced transcripts encoding distinct isoforms have been described.,Mitogen-activated protein kinase kinase kinase 4

Gene ID: 4216

UniProt: Q9Y6R4

Pathways: MAPK Signaling