MAPKAP Kinase 5 antibody (pThr182)

Catalog No. ABIN7225536

This Rabbit Polyclonal antibody specifically detects MAPKAP Kinase 5 in WB, ELISA, IHC and IF. It exhibits reactivity toward Human and Mouse.

Quick Overview for MAPKAP Kinase 5 antibody (pThr182) (ABIN7225536)

Target: MAPKAP Kinase 5 (MAPKAPK5) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 5 (MAPKAPK5))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAPKAP Kinase 5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-MAPKAP Kinase 5 Antibody

Binding Specificity: pThr182

Purpose: Rabbit Anti-PRAK (phospho Thr182) Polyclonal Antibody

Specificity: Phospho-PRAK (T182) Polyclonal Antibody detects endogenous levels of PRAK protein only when phosphorylated at T182.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human PRAK Phospho-Thr182

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:5000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for MAPKAP Kinase 5

Target: MAPKAP Kinase 5 (MAPKAPK5) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 5 (MAPKAPK5))

Alternative Name: PRAK

Background: MAPKAPK5, PRAK, MAP kinase-activated protein kinase 5, MAPK-activated protein kinase 5, MAPKAP kinase 5, MAPKAP-K5, MAPKAPK-5, MK-5, MK5, p38-regulated/activated protein kinase, PRAKThe protein encoded by MAPKAPK5 (mitogen-activated protein kinase-activated protein kinase 5) is a tumor suppressor and member of the serine/threonine kinase family. In response to cellular stress and proinflammatory cytokines, this kinase is activated through its phosphorylation by MAP kinases including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta. The encoded protein is found in the nucleus but translocates to the cytoplasm upon phosphorylation and activation. This kinase phosphorylates heat shock protein HSP27 at its physiologically relevant sites. Two alternately spliced transcript variants of MAPKAPK5 encoding distinct isoforms have been reported.

Molecular Weight: 60kD

Gene ID: 8550

UniProt: Q8IW41

Pathways: MAPK Signaling