NEK9 antibody

Catalog No. ABIN7225860

The Rabbit Polyclonal anti-NEK9 antibody is suitable to detect NEK9 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA and IF.

Quick Overview for NEK9 antibody (ABIN7225860)

Target: NEK9 (NIMA (Never in Mitosis Gene A)- Related Kinase 9 (NEK9))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NEK9 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Product Details anti-NEK9 Antibody

Purpose: Rabbit Anti-Nek9 Polyclonal Antibody

Specificity: Nek9 Polyclonal Antibody detects endogenous levels of Nek9 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human Nek9 around the non-phosphorylation site of T210

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IF 1:200-1:1000,ELISA 1:20000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for NEK9

Target: NEK9 (NIMA (Never in Mitosis Gene A)- Related Kinase 9 (NEK9))

Alternative Name: Nek9

Background: NEK9, KIAA1995, NEK8, NERCC, Serine/threonine-protein kinase Nek9, Nercc1 kinase, Never in mitosis A-related kinase 9, NimA-related protein kinase 9, NimA-related kinase 8, Nek8NEK9 (NIMA related kinase 9) encodes a member of the NimA (never in mitosis A) family of serine/threonine protein kinases. The encoded protein is activated in mitosis and, in turn, activates other family members during mitosis. This protein also mediates cellular processes that are essential for interphase progression.

Molecular Weight: 107kD

Gene ID: 91754

UniProt: Q8TD19

Pathways: SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection