NF-kB p65 antibody (acLys314, Lys315)

Catalog No. ABIN7212366

This Mouse Monoclonal antibody specifically detects NF-kB p65 in IHC and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for NF-kB p65 antibody (acLys314, Lys315) (ABIN7212366)

Target: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This NF-kB p65 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: 2A11

Product Details anti-NF-kB p65 Antibody

Binding Specificity: acLys314, Lys315

Purpose: Mouse Anti-Acetyl NF kB P65 (K314/K315) Mouse Monoclonal Antibody (2A11)

Specificity: The antibody detects endogenous Acetyl NF kB P65 (K314/K315) protein.

Purification: The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthetic Peptide of Acetyl NF kB P65 (K314/K315)

Isotype: IgG1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC 1:50-300,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for NF-kB p65

Target: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Alternative Name: NF kB P65

Background: Transcription factor p65, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA (RELA proto-oncogene, NF-kB subunit), or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of RELA, RELA. Four transcript variants encoding different isoforms have been found for RELA.

Molecular Weight: 65kD

Gene ID: 5970

UniProt: Q04206

Pathways: NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades, S100 Proteins