Peroxiredoxin 3 antibody (Internal Region)

Catalog No. ABIN7216668

The Rabbit Polyclonal anti-Peroxiredoxin 3 antibody is suitable to detect Peroxiredoxin 3 in samples from Human, Rat and Mouse. It has been validated for WB, IHC, ELISA, IF and IP.

Quick Overview for Peroxiredoxin 3 antibody (Internal Region) (ABIN7216668)

Target: Peroxiredoxin 3 (PRDX3)

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Peroxiredoxin 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP)

Product Details anti-Peroxiredoxin 3 Antibody

Binding Specificity: Internal Region

Purpose: Rabbit Anti-PRX III Polyclonal Antibody

Specificity: PRX III Polyclonal Antibody detects endogenous levels of PRX III protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human PRX III

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC:1:50-300,IP 1:50-200,ELISA 1:20000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Peroxiredoxin 3

Target: Peroxiredoxin 3 (PRDX3)

Alternative Name: PRX III

Background: PRDX3, AOP1, Thioredoxin-dependent peroxide reductase, mitochondrial, Antioxidant protein 1, AOP-1, HBC189, Peroxiredoxin III, Prx-III, Peroxiredoxin-3, Protein MER5 homologPRDX3 encodes a mitochondrial protein with antioxidant function. Peroxiredoxin 3 is similar to the C22 subunit of Salmonella typhimurium alkylhydroperoxide reductase, and it can rescue bacterial resistance to alkylhydroperoxide in E.coli that lack the C22 subunit. The human and mouse genes are highly conserved, and they map to the regions syntenic between mouse and human chromosomes. Sequence comparisons with recently cloned mammalian homologs suggest that these genes consist of a family that is responsible for the regulation of cellular proliferation, differentiation and antioxidant functions. This family member can protect cells from oxidative stress, and it can promote cell survival in prostate cancer. Alternative splicing of PRDX3 results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 1, 3, 13 and 22.

Molecular Weight: 26kD

Gene ID: 10935

UniProt: P30048

Pathways: Ribonucleoside Biosynthetic Process, Methionine Biosynthetic Process