PFKM antibody (Internal Region)

Catalog No. ABIN7216508

The Rabbit Polyclonal anti-PFKM antibody has been validated for WB, ELISA and IHC (p). It is suitable to detect PFKM in samples from Human, Mouse and Rat.

Quick Overview for PFKM antibody (Internal Region) (ABIN7216508)

Target: PFKM (phosphofructokinase, Muscle (PFKM))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PFKM antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PFKM Antibody

Binding Specificity: Internal Region

Purpose: PFKM Polyclonal Antibody

Specificity: PFKM Polyclonal Antibody detects endogenous levels of PFKM protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of human PFKM

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:10000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for PFKM

Target: PFKM (phosphofructokinase, Muscle (PFKM))

Alternative Name: PFKM

Background: Rabbit Anti-PFKM Polyclonal Antibody,PFKM, PFKX, 6-phosphofructokinase, muscle type, Phosphofructo-1-kinase isozyme A, PFK-A, Phosphofructokinase-M, Phosphofructokinase 1, Phosphohexokinase,Three phosphofructokinase isozymes exist in humans: muscle, liver and platelet. These isozymes function as subunits of the mammalian tetramer phosphofructokinase, which catalyzes the phosphorylation of fructose-6-phosphate to fructose-1,6-bisphosphate. Tetramer composition varies depending on tissue type. PFKM encodes the muscle-type isozyme. Mutations in PFKM have been associated with glycogen storage disease type VII, also known as Tarui disease. Alternatively spliced transcript variants have been described.,6-phosphofructokinase muscle type

Molecular Weight: observerd band 85kDa

Gene ID: 5213

UniProt: P08237

Pathways: Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Warburg Effect