PIM1 antibody

Catalog No. ABIN7223148

The Rabbit Polyclonal anti-PIM1 antibody (ABIN7223148) specifically detects PIM1 in WB and IHC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for PIM1 antibody (ABIN7223148)

Target: PIM1 (Pim-1 Oncogene (PIM1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIM1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-PIM1 Antibody

Purpose: Rabbit Anti-Pim-1 Polyclonal Antibody

Specificity: Pim-1 Polyclonal Antibody detects endogenous levels of Pim-1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human Pim-1 around the non-phosphorylation site of Y309

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,IHC 1:50-300

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for PIM1

Target: PIM1 (Pim-1 Oncogene (PIM1))

Alternative Name: Pim-1

Background: PIM1, Serine/threonine-protein kinase pim-1The protein encoded by PIM1 (Pim-1 proto-oncogene, serine/threonine kinase) belongs to the Ser/Thr protein kinase family, and PIM subfamily. PIM1 is expressed primarily in B-lymphoid and myeloid cell lines, and is overexpressed in hematopoietic malignancies and in prostate cancer. It plays a role in signal transduction in blood cells, contributing to both cell proliferation and survival, and thus provides a selective advantage in tumorigenesis. Both the human and orthologous mouse genes have been reported to encode two isoforms (with preferential cellular localization) resulting from the use of alternative in-frame translation initiation codons, the upstream non-AUG (CUG) and downstream AUG codons.

Molecular Weight: 50kD

Gene ID: 5292

UniProt: P11309

Pathways: Glycosaminoglycan Metabolic Process