PDGFRA antibody (pTyr849)

Catalog No. ABIN7223040

The Rabbit Polyclonal anti-PDGFRA antibody (ABIN7223040) specifically detects PDGFRA in WB, ELISA and IHC (p). The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for PDGFRA antibody (pTyr849) (ABIN7223040)

Target: PDGFRA (Platelet Derived Growth Factor Receptor alpha (PDGFRA))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDGFRA antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PDGFRA Antibody

Binding Specificity: pTyr849

Purpose: PDGFR-α (phospho Tyr849) Polyclonal Antibody

Specificity: Phospho-PDGFR-α (Y849) Polyclonal Antibody detects endogenous levels of PDGFR-α protein only when phosphorylated at Y849.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human PDGFR-alpha Phospho-Tyr849

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:5000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for PDGFRA

Target: PDGFRA (Platelet Derived Growth Factor Receptor alpha (PDGFRA))

Alternative Name: PDGFR-alpha

Background: Rabbit Anti-PDGFR-α (phospho Tyr849) Polyclonal Antibody,PDGFRA, PDGFR2, RHEPDGFRA, Platelet-derived growth factor receptor alpha, PDGF-R-alpha, PDGFR-alpha, Alpha platelet-derived growth factor receptor, Alpha-type platelet-derived growth factor receptor, CD140 antigen-like family member A, CD14,PDGFRA (platelet derived growth factor receptor alpha) encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies suggest that this gene plays a role in organ development, wound healing, and tumor progression. Mutations in PDGFRA have been associated with idiopathic hypereosinophilic syndrome, somatic and familial gastrointestinal stromal tumors, and a variety of other cancers.,Platelet-derived growth factor receptor alpha

Molecular Weight: observerd band 140kDa

Gene ID: 5156

UniProt: P16234

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Platelet-derived growth Factor Receptor Signaling