POLL antibody (AA 50-130)

Catalog No. ABIN7214592

This Rabbit Polyclonal antibody specifically detects POLL in WB and ELISA. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for POLL antibody (AA 50-130) (ABIN7214592)

Target: POLL (Polymerase (DNA Directed), lambda (POLL))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLL antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-POLL Antibody

Binding Specificity: AA 50-130

Purpose: Rabbit Anti-DNA pol α Polyclonal Antibody

Specificity: DNA pol α Polyclonal Antibody detects endogenous levels of DNA pol α protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the N-terminal region of human DNA pol alpha at AA range: 50-130

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,ELISA 1:20000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for POLL

Target: POLL (Polymerase (DNA Directed), lambda (POLL))

Alternative Name: DNA pol alpha

Background: POLA1, POLA, DNA polymerase alpha catalytic subunit, DNA polymerase alpha catalytic subunit p180POLA1 encodes the catalytic subunit of DNA polymerase, which together with a regulatory and two primase subunits, forms the DNA polymerase alpha complex. The catalytic subunit plays an essential role in the initiation of DNA replication.

Molecular Weight: 165kD

Gene ID: 5422

UniProt: P09884

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response