PAPPA antibody (AA 30-110)

Catalog No. ABIN7230486

This Rabbit Polyclonal antibody specifically detects PAPPA in WB and ELISA. It exhibits reactivity toward Human and Mouse.

Quick Overview for PAPPA antibody (AA 30-110) (ABIN7230486)

Target: PAPPA (Pregnancy-Associated Plasma Protein A, Pappalysin 1 (PAPPA))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAPPA antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-PAPPA Antibody

Binding Specificity: AA 30-110

Purpose: Rabbit Anti-PAPP1 Polyclonal Antibody

Specificity: The antibody detects endogenous levels of PAPP1 protein

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from part region of human PAPP1 protein at AA range: 30-110

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,ELISA 1:5000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 %BSA

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for PAPPA

Target: PAPPA (Pregnancy-Associated Plasma Protein A, Pappalysin 1 (PAPPA))

Alternative Name: PAPP1

Background: Insulin-like growth factor-dependent IGF-binding protein 4 protease, IGF-dependent IGFBP-4 protease, IGFBP-4ase, Pregnancy-associated plasma protein A, PAPP-APAPPA encodes a secreted metalloproteinase which cleaves insulin-like growth factor binding proteins (IGFBPs). Following IGFBP cleavage, insulin growth factors dissociate from IGFBPs and bind to IGF receptors, resulting in activation of the IGF pathway. The encoded protein plays a role in bone formation, inflammation, wound healing and female fertility. Enhanced expression of this protein is associated with diabetic nephropathy in human patients and this protein may promote tumor invasion and growth in various human cancers.

Molecular Weight: 178kD

Gene ID: 5069

UniProt: Q13219