PKA antibody (AA 1-80)

Catalog No. ABIN7216582

The Rabbit Polyclonal anti-PKA antibody is suitable to detect PKA in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IF and IHC.

Quick Overview for PKA antibody (AA 1-80) (ABIN7216582)

Target: PKA (Protein Kinase A (PKA))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKA antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC)

Product Details anti-PKA Antibody

Binding Specificity: AA 1-80

Purpose: Rabbit Anti-PKAα/β cat Polyclonal Antibody

Specificity: PKAα/β cat Polyclonal Antibody detects endogenous levels of PKAα/β cat protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the N-terminal region of human PKAalpha/beta cat at AA range: 1-80

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:20000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for PKA

Target: PKA (Protein Kinase A (PKA))

Alternative Name: PKAalpha/beta

Background: PRKACA, PKACA, cAMP-dependent protein kinase catalytic subunit alpha, PKA C-alpha, PRKACB, cAMP-dependent protein kinase catalytic subunit beta, PKA C-betaPRKACA encodes one of the catalytic subunits of protein kinase A, which exists as a tetrameric holoenzyme with two regulatory subunits and two catalytic subunits, in its inactive form. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. cAMP-dependent phosphorylation of proteins by protein kinase A is important to many cellular processes, including differentiation, proliferation, and apoptosis. Constitutive activation of PRKACA caused either by somatic mutations, or genomic duplications of regions that include PRKACA, have been associated with hyperplasias and adenomas of the adrenal cortex and are linked to corticotropin-independent Cushing's syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. Tissue-specific isoforms that differ at the N-terminus have been described, and these isoforms may differ in the post-translational modifications that occur at the N-terminus of some isoforms.

Molecular Weight: 38kD

Gene ID: 5566, 5567

UniProt: P17612, P22694

Pathways: SARS-CoV-2 Protein Interactome