POFUT1 antibody (AA 300-380)

Catalog No. ABIN7220730

The Rabbit Polyclonal anti-POFUT1 antibody (ABIN7220730) specifically detects POFUT1 in WB, IHC, ELISA and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for POFUT1 antibody (AA 300-380) (ABIN7220730)

Target: POFUT1 (Protein O-Fucosyltransferase 1 (POFUT1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POFUT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-POFUT1 Antibody

Binding Specificity: AA 300-380

Purpose: Rabbit Anti-O-FucT-1 Polyclonal Antibody

Specificity: O-FucT-1 Polyclonal Antibody detects endogenous levels of O-FucT-1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the C-terminal region of human O-FucT-1 at AA range: 300-380

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:40000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for POFUT1

Target: POFUT1 (Protein O-Fucosyltransferase 1 (POFUT1))

Alternative Name: O-FucT-1

Background: POFUT1, FUT12, KIAA0180, GDP-fucose protein O-fucosyltransferase 1, Peptide-O-fucosyltransferase 1, O-FucT-1POFUT1 encodes a member of the glycosyltransferase O-Fuc family. This enzyme adds O-fucose through an O-glycosidic linkage to conserved serine or threonine residues in the epidermal growth factor-like repeats of a number of cell surface and secreted proteins. O-fucose glycans are involved in ligand-induced receptor signaling. Alternative splicing of this gene results in two transcript variants encoding different isoforms.

Molecular Weight: 44kD

Gene ID: 23509

UniProt: Q9H488

Pathways: Notch Signaling, SARS-CoV-2 Protein Interactome