CD45 antibody (pSer1007)

Catalog No. ABIN7223842

This Rabbit Polyclonal antibody specifically detects CD45 in WB, IF and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for CD45 antibody (pSer1007) (ABIN7223842)

Target: CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD45 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA

Product Details anti-CD45 Antibody

Binding Specificity: pSer1007

Purpose: CD45 (phospho Ser1007) Polyclonal Antibody

Specificity: Phospho-CD45 (S1007) Polyclonal Antibody detects endogenous levels of CD45 protein only when phosphorylated at S1007.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human CD45 Phospho-Ser1007

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IF (1:200-1:1000), ELISA (1:5000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for CD45

Target: CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))

Alternative Name: CD45

Background: Rabbit Anti-CD45 (phospho Ser1007) Polyclonal Antibody,PTPRC, CD45, Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, L-CA, T200, CD antigen CD45,The protein encoded by PTPRC (protein tyrosine phosphatase, receptor type C) is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of PTPRC, which encode distinct isoforms, have been reported.,Receptor-type tyrosine-protein phosphatase C

Molecular Weight: observerd band 150kDa

Gene ID: 5788

UniProt: P08575

Pathways: TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, BCR Signaling