RACGAP1 antibody

Catalog No. ABIN7216734

This Rabbit Polyclonal antibody specifically detects RACGAP1 in WB, IHC, ELISA and IF. It exhibits reactivity toward Human, Mouse, Rat and Monkey.

Quick Overview for RACGAP1 antibody (ABIN7216734)

Target: RACGAP1 (Rac GTPase Activating Protein 1 (RACGAP1))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RACGAP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-RACGAP1 Antibody

Purpose: Rabbit Anti-Rac GAP1 Polyclonal Antibody

Specificity: Rac GAP1 Polyclonal Antibody detects endogenous levels of Rac GAP1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human Rac GAP1 around the non-phosphorylation site of S387

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:40000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for RACGAP1

Target: RACGAP1 (Rac GTPase Activating Protein 1 (RACGAP1))

Alternative Name: Rac GAP1

Background: RACGAP1, KIAA1478, MGCRACGAP, Rac GTPase-activating protein 1, Male germ cell RacGap, MgcRacGAP, Protein CYK4 homolg, CYK4, HsCYK-4RACGAP1 encodes a GTPase-activating protein (GAP) that is a compoment of the centralspindlin complex. Rac GTPase activating protein 1 binds activated forms of Rho GTPases and stimulates GTP hydrolysis, which results in negative regulation of Rho-mediated signals. Rac GTPase activating protein 1 plays a regulatory role in cytokinesis, cell growth, and differentiation. Alternatively spliced transcript variants have been found for RACGAP1. There is a pseudogene for RACGAP1 on chromosome 12.

Molecular Weight: 72kD

Gene ID: 29127

UniProt: Q9H0H5

Pathways: Regulation of Actin Filament Polymerization, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, Signaling of Hepatocyte Growth Factor Receptor