Retinoblastoma Binding Protein 8 antibody

Catalog No. ABIN7223950

The Rabbit Polyclonal anti-Retinoblastoma Binding Protein 8 antibody is suitable to detect Retinoblastoma Binding Protein 8 in samples from Human and Mouse. It has been validated for ELISA, WB, IHC and IF.

Quick Overview for Retinoblastoma Binding Protein 8 antibody (ABIN7223950)

Target: Retinoblastoma Binding Protein 8 (RBBP8)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Retinoblastoma Binding Protein 8 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-Retinoblastoma Binding Protein 8 Antibody

Purpose: Rabbit Anti-CtIP Polyclonal Antibody

Specificity: CtIP Polyclonal Antibody detects endogenous levels of CtIP protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human CtIP around the non-phosphorylation site of S327

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,IHC 1:100-1:300,ELISA 1:40000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Retinoblastoma Binding Protein 8

Target: Retinoblastoma Binding Protein 8 (RBBP8)

Alternative Name: CtIP

Background: RBBP8, CTIP, DNA endonuclease RBBP8, CtBP-interacting protein, CtIP, Retinoblastoma-binding protein 8, RBBP-8, Retinoblastoma-interacting protein and myosin-like, RIM, Sporulation in the absence of SPO11 protein 2 homolog, SAE2The protein encoded by RBBP8 (RB binding protein 8, endonuclease) is a ubiquitously expressed nuclear protein. It is found among several proteins that bind directly to retinoblastoma protein, which regulates cell proliferation. This protein complexes with transcriptional co-repressor CTBP. It is also associated with BRCA1 and is thought to modulate the functions of BRCA1 in transcriptional regulation, DNA repair, and/or cell cycle checkpoint control. It is suggested that RBBP8 may itself be a tumor suppressor acting in the same pathway as BRCA1. Three transcript variants encoding two different isoforms have been found for RBBP8. More transcript variants exist, but their full-length natures have not been determined.

Molecular Weight: 100kD

Gene ID: 5932

UniProt: Q99708

Pathways: Cell Division Cycle, DNA Damage Repair