MSK1 antibody (pSer376)

Catalog No. ABIN7213190

The Rabbit Polyclonal anti-MSK1 antibody (ABIN7213190) specifically detects MSK1 in WB and IHC. The antibody is reactive with Human and Mouse samples.

Quick Overview for MSK1 antibody (pSer376) (ABIN7213190)

Target: MSK1 (RPS6KA5) (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 5 (RPS6KA5))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MSK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-MSK1 Antibody

Binding Specificity: pSer376

Purpose: Rabbit Anti-MSK1 (phospho Ser376) Polyclonal Antibody

Specificity: Phospho-MSK1 (S376) Polyclonal Antibody detects endogenous levels of MSK1 protein only when phosphorylated at S376.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human MSK1 Phospho-Ser376

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,IHC 1:50-300

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for MSK1

Target: MSK1 (RPS6KA5) (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 5 (RPS6KA5))

Alternative Name: MSK1

Background: RPS6KA5, MSK1, Ribosomal protein S6 kinase alpha-5, S6K-alpha-5, 90 kDa ribosomal protein S6 kinase 5, Nuclear mitogen- and stress-activated protein kinase 1, RSK-like protein kinase, RSKLMSK-1 is a mitogen and stress activated protein kinase-1 which belongs to the AGC family of kinases and is related in structure to the ribosomal p70 S6 kinase subfamily. MSK-1can be activated by ERK1/2 and SAPK2 /p38 MAP kinase. It is also known to be required for the phosphorylation of CREB, ATF1 H3 and HMG-14 in response to mitogen and stress. Similar to RSK, MSK-1 contains two kinase domains (N-term and a C-term). Once phosphorylated on Thr581 and Ser360 by ERK1/2 and SAPK2/p38, MSK-1 autophosphorylate on at least 5 sites. Of these autophosphorylation sites Ser212 and Ser376 get phosphorylated by the C-terminal kinase domain of MSK-1 which is essential for the catalytic activity of the N-terminal kinase domain.

Molecular Weight: 90kD

Gene ID: 9252

UniProt: O75582

Pathways: MAPK Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades