RYK antibody (AA 150-230)

Catalog No. ABIN7231216

The Rabbit Polyclonal anti-RYK antibody (ABIN7231216) specifically detects RYK in WB and ELISA. The antibody is reactive with Human and Mouse samples.

Quick Overview for RYK antibody (AA 150-230) (ABIN7231216)

Target: RYK (RYK Receptor-Like Tyrosine Kinase (RYK))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RYK antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-RYK Antibody

Binding Specificity: AA 150-230

Purpose: Rabbit Anti-RYK Polyclonal Antibody

Specificity: The antibody detects endogenous levels of RYK protein

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from part region of human RYK protein at AA range: 150-230

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,ELISA 1:5000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 %BSA

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for RYK

Target: RYK (RYK Receptor-Like Tyrosine Kinase (RYK))

Alternative Name: RYK

Background: Tyrosine-protein kinase RYKThe protein encoded by RYK is an atypical member of the family of growth factor receptor protein tyrosine kinases, differing from other members at a number of conserved residues in the activation and nucleotide binding domains. This gene product belongs to a subfamily whose members do not appear to be regulated by phosphorylation in the activation segment. It has been suggested that mediation of biological activity by recruitment of a signaling-competent auxiliary protein may occur through an as yet uncharacterized mechanism. The encoded protein has a leucine-rich extracellular domain with a WIF-type Wnt binding region, a single transmembrane domain, and an intracellular tyrosine kinase domain. This protein is involved in stimulating Wnt signaling pathways such as the regulation of axon pathfinding. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Molecular Weight: 66kD

Gene ID: 6259

UniProt: P34925

Pathways: RTK Signaling, WNT Signaling, Regulation of Cell Size