SGK1 antibody (pSer78)

Catalog No. ABIN7224280

The Rabbit Polyclonal anti-SGK1 antibody is suitable to detect SGK1 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA and IHC.

Quick Overview for SGK1 antibody (pSer78) (ABIN7224280)

Target: SGK1 (serum/glucocorticoid Regulated Kinase 1 (SGK1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SGK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-SGK1 Antibody

Binding Specificity: pSer78

Purpose: Rabbit Anti-SGK1 (phospho Ser78) Polyclonal Antibody

Specificity: Phospho-SGK1 (S78) Polyclonal Antibody detects endogenous levels of SGK1 protein only when phosphorylated at S78.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human SGK1 Phospho-Ser78

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,IHC 1:50-300,ELISA 1:2000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for SGK1

Target: SGK1 (serum/glucocorticoid Regulated Kinase 1 (SGK1))

Alternative Name: SGK1

Background: SGK1, SGK, Serine/threonine-protein kinase Sgk1, Serum/glucocorticoid-regulated kinase 1SGK1 (serum/glucocorticoid regulated kinase 1) encodes a serine/threonine protein kinase that plays an important role in cellular stress response. This kinase activates certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion. High levels of expression of SGK1 may contribute to conditions such as hypertension and diabetic nephropathy. Several alternatively spliced transcript variants encoding different isoforms have been noted for SGK1.

Molecular Weight: 54kD

Gene ID: 6446

UniProt: O00141

Pathways: MAPK Signaling, Notch Signaling, Steroid Hormone Mediated Signaling Pathway